Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Assessment of PAS effects on planktonic K. quasipneumoniae ATCC 700603. A – E Bacterial growth curves under different treatment conditions (as indicated in the diagrams). The concentrations of CAZ as the fold change in the MIC (MIC CAZ = 32 mg/L) and phage titers are indicated in the legends. The data are presented as the means ± standard deviations (SDs, n = 3)

Article Snippet: The Klebsiella quasipneumoniae subsp. similipneumoniae strain ATCC 700603 (GenBank accession number CP014696.2 ) used in this study was originally isolated from the urine of a hospitalized patient and was formerly classified as Klebsiella pneumoniae .

Techniques:

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Quantitative evaluation of treatment effects on K. quasipneumoniae ATCC 700603 biofilms following exposure to CAZ (0.25 × MIC), phage vB_KpUKJ_2 (10 8 PFU/mL), or their combination. A Surface area covered (SAC) by viable biofilm (in %) over time, as determined by the calcein fluorescence signal after staining with CAM. A total pixel area of 2048 × 2048 µm was analyzed from LSFM micrographs; B AUC representation of the kinetic data from ( A ) over a treatment period of 0–24 h, based on relative fluorescence units (RFU) × h; C Viable bacterial counts (CFU/mL) after biofilm treatments at three distinct time points. Statistical analysis was performed via two-way ANOVA followed by Tukey’s multiple comparisons test. D Phage particles (PFU/mL) after combined biofilm treatment at three time points. Statistical analysis was conducted via one-way ANOVA followed by Dunnett’s post hoc test. The data are presented as the means ± SDs (n = 3). Significance was assumed at p < 0.05 and is indicated by asterisks: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Article Snippet: The Klebsiella quasipneumoniae subsp. similipneumoniae strain ATCC 700603 (GenBank accession number CP014696.2 ) used in this study was originally isolated from the urine of a hospitalized patient and was formerly classified as Klebsiella pneumoniae .

Techniques: Fluorescence, Staining

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Representative CLSM images showing the effects of phage vB_KpUKJ_2 (10 8 PFU/mL) on mature K. quasipneumoniae ATCC 700603 biofilms at 0, 4, and 24 h post-treatment. Biofilms were stained with Con-A (green) for α-polysaccharides or with CFW (magenta) for β-polysaccharides to visualize extracellular matrix integrity. A Untreated control, B phage-treated samples. To minimize photobleaching and laser-induced biofilm disruption, independent samples were used for each time point. Each image represents a representative Z-stack projection from three independent experiments. Scale bars: 20 μm

Article Snippet: The Klebsiella quasipneumoniae subsp. similipneumoniae strain ATCC 700603 (GenBank accession number CP014696.2 ) used in this study was originally isolated from the urine of a hospitalized patient and was formerly classified as Klebsiella pneumoniae .

Techniques: Staining, Control, Disruption

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Characteristics of six phage-resistant mutants in comparison with the parental K. quasipneumoniae ATCC 700603 strain. A Differences in the AUCs based on growth kinetics. B Biofilm formation ability at 48 h quantified by crystal violet staining and absorbance measured at 570 nm; the data are presented as the means ± SDs (n = 3). Statistical analyses were performed via one-way ANOVA followed by Tukey’s multiple comparisons test. Significance was assumed at p < 0.05 and is indicated by asterisks: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0. 0001

Article Snippet: The Klebsiella quasipneumoniae subsp. similipneumoniae strain ATCC 700603 (GenBank accession number CP014696.2 ) used in this study was originally isolated from the urine of a hospitalized patient and was formerly classified as Klebsiella pneumoniae .

Techniques: Comparison, Staining

Journal: Journal of Biological Inorganic Chemistry

Article Title: Antibacterial activity of metal–phenanthroline complexes against multidrug-resistant Irish clinical isolates: a whole genome sequencing approach

doi: 10.1007/s00775-022-01979-8

Figure Lengend Snippet: Disc-diffusion antimicrobial susceptibility testing using common antibacterial agents

Article Snippet: The strains representing the test Enterobacterales group were ATCC 700603, ATCC BAA1705, ESBL1, MBL1 and KPC1 isolates (Table S 7).

Techniques:

Journal: Journal of Biological Inorganic Chemistry

Article Title: Antibacterial activity of metal–phenanthroline complexes against multidrug-resistant Irish clinical isolates: a whole genome sequencing approach

doi: 10.1007/s00775-022-01979-8

Figure Lengend Snippet: Gram-negative panel. Heat map displaying the mean MIC values of metal complexes, grouped according to their metal centre and also antibiotic controls (meropenem and gentamicin) for ( A ) Enterobacterales control strain ATCC 29213 ( E. coli ), ATCC 10031 ( K. pneumoniae ), ATCC 700603 (ESBL +), ATCC BAA1705 (KPC +) and clinical isolates ESBL1, MBL1 and KPC1, and (B) Pseudomonas aeruginosa , control strain ATCC 27853 and PAO1, and clinical isolates PA1–PA4. Graph tiles that are white without a value, did not have a MIC value that fell within the test range (> 256 µg/mL). Details of the concentration range, and its relation to colour, is displayed in the figure legend

Article Snippet: The strains representing the test Enterobacterales group were ATCC 700603, ATCC BAA1705, ESBL1, MBL1 and KPC1 isolates (Table S 7).

Techniques: Concentration Assay

Journal: Antibiotics

Article Title: Pharmaceutical Prospects of Bee Products: Special Focus on Anticancer, Antibacterial, Antiviral, and Antiparasitic Properties

doi: 10.3390/antibiotics10070822

Figure Lengend Snippet: Antibacterial activity of selected bee products.

Article Snippet: Red propolis, green propolis, and brown propolis (traditionally extracted using ethanol or supercritical extraction method) , Brazil , S. aureus (ATCC 25923) and Enterococcus sp. (ATCC 29712), Klebsiella sp. (ATCC 1706/700603), and E. coli (ATCC 25922) , In vitro (micro-dilution assay) , Of all samples, Brazilian red propolis extract yielded the highest antibacterial activity. Green propolis extract demonstrated weak to moderate antibacterial activity for most samples and brown propolis extract did not yield any antibacterial effect against the tested strains. None of the examined samples was active against E. coli , [ ] .

Techniques: Activity Assay, Bacteria, Derivative Assay, In Vitro, Diffusion-based Assay, Dilution Assay, Microdilution Assay, Extraction, Transgenic Assay, Luciferase, Control, Biofilm Production Assay, Concentration Assay, SYBR Green Assay, Viability Assay, Membrane, Permeability, Isolation